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The expression of Cat C mRNA and protein in corpus callosum in wild type and Cys F KO mice treated by cuprizone for 4 weeks. Cat C ISH (A–C) and Cat C IHC (D–F) staining were performed in the untreated mice (A,D), cuprizone-treated wild type (B,E) and Cys F KO mice (C,F). Percentages of Cat C ISH and Cat C IHC positive cells in corpus callosum are shown in (G,H), respectively. *P < 0.05, **P < 0.01. n = 3 per group. Scale bar in (A–F): 200 μm.
