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. 2016 Dec 21;9:152. doi: 10.3389/fnmol.2016.00152

Figure 3.

Figure 3

The accumulation of microglia/macrophagesin corpus callosum in wild type and Cys F KO mice after cuprizone treatment for 4 weeks. Iba-1 IHC (A–C) and c-fms ISH (D–F) staining were performed in untreated mice (A,D), 4 week-cuprizone treated wild type (B,E) and Cys F KO mice (C,F). The double staining of Cat C IHC and Iba-1 IHC staining is seen in (G). Quantitative analysis result of c-fms positive cells in corpus callosum is shown in (H). *P < 0.05, **P < 0.01. n = 4 per group. Scale bar in (A–F): 200 μm.