Figure 4.

CD45 positive cells in wild type and Cys F KO mice treated by cuprizone for 4 weeks. CD45 IHC was performed in untreated wild type mice (A), 4 week- cuprizone treated wild type (B) and Cys F KO mice (C), respectively. Quantitative analysis of CD45 positive cells in corpus callosum is shown in (D). Iba-1 and CD45 immunofluorescence staining is shown in (E–G), the arrows showed Iba-1 and CD45 double staining cells. ^*P < 0.05, ^**P < 0.01. n = 4 per group. Scale bar in (A–C): (E) 200 μm; in (E) 10 μm.