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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Toxicon. 2016 Sep 15;122:43–49. doi: 10.1016/j.toxicon.2016.09.007

Fig. 3. SDS-PAGE of r-colombistatins 2, 3, and 4.

Fig. 3

Samples (3 μg) were run on 4–12% (w/v) Bis-Tris Gel using an Xcell SureLock Mini-Cell at 200 V for 30 min. The gel was stained with RapidStain. Lane 1: SeeBlue Plus2 Markers; lane 2: purified r-colombistatin 2; lane 3: purified r-colombistatin 3; lane 4: purified r-colombistatin 4. An asterisk (*) represents the N-terminal amino acid sequences of purified r-colombistatins containing the five amino acids from the vector (italicized) before the disintegrin-like sequences, which are shown in bold letters.