Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Transplantation. 2016 Dec;100(12):2630–2639. doi: 10.1097/TP.0000000000001465

To determine whether NHP PBMNC could mediate a xeno-GVHD response, PBMNC were prepared from NHP leukapheresis products and were injected (30×10⁶ cells) into NOD/Scid/γc−/− (NSG) ± 200 cGray total body irradiation. Control NSG mice receiving irradiation only were also monitored. Disease progression was monitored by weight loss (A), GVHD score (B) and survival (C). Representative example (D) and summary (E) of xenoGVHD histology of Ileum, liver and lung from irradiated animals with or without NHP PBMNC. (D and E). n=6 mice per group. * denotes days when average weight or clinical score for both fresh and frozen/thawed PBMNC + Irr. Resulted in a p<0.05 compared to Irr. or PBMNC only.

To determine whether NHP PBMNC could mediate a xeno-GVHD response, PBMNC were prepared from NHP leukapheresis products and were injected (30×10⁶ cells) into NOD/Scid/γc−/− (NSG) ± 200 cGray total body irradiation. Control NSG mice receiving irradiation only were also monitored. Disease progression was monitored by weight loss (A), GVHD score (B) and survival (C). Representative example (D) and summary (E) of xenoGVHD histology of Ileum, liver and lung from irradiated animals with or without NHP PBMNC. (D and E). n=6 mice per group. * denotes days when average weight or clinical score for both fresh and frozen/thawed PBMNC + Irr. Resulted in a p<0.05 compared to Irr. or PBMNC only.