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TSH and CD40L induced IL-12 at the pre-translational level. Steady-state IL-12p40 mRNA expression stimulated with TSH (▲) or CD40L (■) was followed by real-time polymerase chain reaction (PCR) in cultured fibrocytes for 24 h (A). IL-12p40 mRNA production increased responding to TSH, M22, and CD40L stimulation in fibrocytes from healthy controls (B, left panel) and from patients with GD (B, right panel).
