Table 1.

Akt and NF-κB Are Involved in TSHR and CD40 Signaling for IL-12 Production in Circulating Fibrocytes

	IL-12 p40/p70 (MFI)	IL-12 p70 (MFI)
Control	3.56	1.59
TSH	5.09	3.87
TSH + AKTi	2.81	2.37
TSH + MG132	1.99	1.74
CD40L	4.11	3.71
CD40L + AKTi	2.69	2.16
CD40L + MG132	2.06	1.69

Intracellular IL-12 p40/p70 (combined monomer and heterodimer) and IL-12 p70 (heterodimer) were measured by flow cytometry 18 h after TSH or CD40L was added. Akt inhibitor (AKTi) and NF-κB inhibitor (MG132) were added to cells 1 h before stimulation. TSH or CD40L stimulation was markedly reduced by adding AKTi or MG132.

TSHR, thyrotropin receptor; IL-12, interleukin-12; MFI, mean fluorescent intensity; TSH, thyrotropin.