TABLE 1.
Pneumocystis burdens in lungs of mice
| Mouse model | Time point (days postinoculation) | Log10 no.a of Pneumocystis nuclei per lung (no. of mice) in:
|
||
|---|---|---|---|---|
| KO (+CD4)b mice | KO (−CD4)c mice | WT (−CD4)d mice | ||
| CXCR2-KO | 15 | NDe | 7.01 ± 0.22 (4) | 6.96 ± 0.91 (5) |
| 21 | ND | 7.06 ± 0.31 (6) | 7.66 ± 0.24 (5) | |
| 35 | ND | 7.38 ± 0.75 (3) | 7.76 ± 0.21 (5) | |
| 15 | ND | 6.31 ± 0.31 (5) | 6.26 ± 0.49 (5) | |
| gp91phox-KO | 28 | ND | 7.77 ± 0.70 (3) | 7.94 ± 0.08 (3) |
| 35 | 4.43 ± 0.00 (5) | 7.96 ± 0.31 (5) | 7.76 ± 0.60 (5) | |
| phox/iNOS-KO | 21 | 4.98 ± 0.72 (4) | 6.96 ± 0.08 (3) | 6.46 ± 0.81 (4) |
| 28 | 4.47 ± 0.45 (4) | 7.77 ± 0.60 (5) | 8.26 ± 0.08 (5) | |
| 35 | 4.91 ± 0.91 (5) | 8.32 ± 0.06 (5) | 8.14 ± 0.14 (5) | |
Values are means ± SD. No Pneumocystis nuclei were found in wild-type mice used as controls that were not depleted of CD4+ lymphocytes or infected with Pneumocystis (n = 5). This result translates to a log 4.40 or 4.24, which is the limit of detection in this method of counting depending on the microscope lens used.
KO (+CD4), knockout mice corresponding to the indicated model in which CD4+ lymphocytes were not depleted but that were inoculated with Pneumocystis.
KO (−CD4), knockout mice corresponding to the indicated model that were depleted of CD4+ lymphocytes during the experiment and inoculated with Pneumocystis.
WT (−CD4), wild-type mice appropriate for the indicated model that were depleted of CD4+ lymphocytes and infected with the same dose as the corresponding knockout mice.
Not done.