FIG. 4.

IGF-1R tyrosine kinase inhibition by Linsitinib (LINS) or PQ-401 mitigates the ability of TSH to stimulate NIS gene expression. (A) Human thyrocytes were treated with the indicated dose of LINS or (B) PQ-401 alone or in combination with 100 mIU/mL of TSH. RNA was isolated after 48 h, cDNA synthesized, and the indicated genes measured by quantitative PCR. Data are expressed as mean ± SEM, n = 4. *p < 0.05, TSH + indicated LINS dose versus TSH alone; ****p < 0.0001, TSH + PQ-401 versus TSH alone. No significant effect of LINS or PQ-401 alone was observed on either gene.