FIG. 8.

The engineered vasculature within the decellularized intestine maintains the capacity for sprouting angiogenesis. HDMECs were perfused through the vascular channels and HDFs were seeded through the intestinal lumen as described in Figs. 6 and 7 before perfusion at 0.025 mL/min for 72 h. Collagen gels with or without VEGFA were placed on top of the intestine. Intestine sections were fixed and stained for CD31 or Dll4 using immunofluorescence whole-mount staining methods. Example images are shown of intestine with and without VEGFA, and regions positive for Dll4 are indicated by arrows. Scale bars are 100 μm. VEGFA, vascular endothelial growth factor A.