Table 1.

Assessment of Extent of Endothelialization in Jejunum with Addition of HDFs and Flow

		Static
Expt No.	+HDFs	1 day	3 days	Perfused (rate mL/min) 3 days
1	0	+++	ND	ND
2	0	++	+	++ (2.7)
3	0	+++	+	++ (2.7)
4	+HDFs (vessel)	++	+	++ (0.025)
5	+HDFs (lumen)	+++	0	+++ (0.025)
6	0	ND	ND	++ (0.025)
	+HDFs (vessel)	ND	ND	+ (0.025)
	+HDFs (lumen)	ND	ND	+++ (0.025)

The presence of endothelial cells was detected using immunostaining for CD31 and photographs were then scored by three observers blind to the nature of the experiment. Observers were asked to score 0 for no evidence of endothelial cell cover, + for some endothelial cells, but poor (<25% cover), ++ for reasonable, but incomplete endothelial cover, and +++ for complete endothelial cover.

HDFs, human dermal fibroblasts; ND, not done.