FIG. 4.

Y-27632 prevents stress fiber formation in cryopreserved iPSC-BMECs. (A) IMR90-4 iPSC-BMECs were cryopreserved at D8 of differentiation and immunostained at D10, 2 days post-thaw. Y-27632 (10 μM) was present D8–D10. (B) IMR90-4 iPSC-BMECs were cryopreserved at D8 of differentiation and immunostained at D12, 4 days post-thaw. Y-27632 (10 μM) was present D8–D12. (C) IMR90-4 iPSC-BMECs were cryopreserved at D8 of differentiation, and phalloidin staining was performed at D12, 4 days post-thaw. Y-27632 (10 μM) was present D8–D12. (A–C) Scale bars, 50 μm. Color images available online at www.liebertpub.com/tec