Immunohistochemical analysis of calvarial defect healing. (A, B) OCN, (C, D) BSP, and (E, F) VEGF expression were interrogated by immunohistochemistry among control- and SAG-treated conditions (1 mM). Black arrowheads indicate immunostaining within osteocytes. Black dashed lines indicate the native bone defect margin among control samples. A single representative sample per treatment group is shown. Scale bar = 50 μm. OCN, osteocalcin; BSP, bone sialoprotein; VEGF, vascular endothelial growth factor. Color images available online at www.liebertpub.com/tea