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Partial protection induced by active immunization. BALB/c mice were immunized three times with purified soluble antigen emulsified with IFA. At 45 days after the last immunization, animals were infected with N. brasiliensis. Results represent means and SDs for 10 animals per group (P = 0.0001). Symbols: ▴, P24 antigen (SD, ±0.66); ♦, P61 antigen (SD, ±0.66); •, P38 antigen (SD, ±0.33); ▪, positive control (SD, ±0.40).
