TABLE 1.
Kinetics of natural polyreactive IgM antibodies and total and antigen-specific IgM responses in BALB/c mice immunized with N. brasiliensis antigens
| Antigen used to immunize mice | Day(s) after immunization | IgM
|
||
|---|---|---|---|---|
| Mean ± SD (n = 10)
| ||||
| Totala | Antigen specificb | Polyreactivec | ||
| P61 | 0 | 2.5 ± 0.6 | 0.114 ± 0.02 | Negative |
| 1 | 3.27 ± 0.8 | 0.111 ± 0.02 | Positive | |
| 3 | 5.04 ± 1.2 | 0.308 ± 0.07 | Positive | |
| 7 | 7.97 ± 1.9 | 0.251 ± 0.06 | Positive | |
| 15 | 6.68 ± 1.6 | 0.136 ± 0.03 | Positive | |
| P38 | 0 | 2.5 ± 0.6 | 0.230 ± 0.05 | Negative |
| 1 | 9.04 ± 2.2 | 0.304 ± 0.07 | Positive | |
| 3 | 125 ± 31 | 0.215 ± 0.05 | Positive | |
| 7 | 9.45 ± 2.3 | 0.330 ± 0.08 | Positive | |
| 15 | 4.54 ± 1.1 | 0.231 ± 0.05 | Positive | |
| P24 | 0 | 2.5 ± 0.6 | 0.093 ± 0.023 | Negative |
| 1 | 6.81 ± 1.7 | 0.101 ± 0.025 | Positive | |
| 3 | 23.3 ± 5.8 | 0.103 ± 0.02 | Positive | |
| 7 | 9.8 ± 2.4 | 0.183 ± 0.04 | Positive | |
| 15 | 6.13 ± 1.5 | 0.135 ± 0.03 | Positive | |
a
Determined by nephelometry. Results are reported in micrograms per milliliter.
b
Determined by an ELISA. Results are reported as absorbance at 492 nm.
c
Determined by hemagglutination of red blood cells.