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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Methods Mol Biol. 2016;1403:499–518. doi: 10.1007/978-1-4939-3387-7_28

Fig. 2.

Fig. 2

Cloning strategy to generate pET-F1mutV plasmid. T7 indicates T7 promoter sequence, MCS indicates multiple cloning site, P1–P7 indicate primers 1–7, blue indicates the first 14-aa residues of F1, and orange indicates aa residues 15–21 of F1. The remaining region of F1 is shown in green. V gene is shown in cyan. The linkers in F1mut or between F1mut and V are not shown