Figure 1.

Metabolism of PCB 95 by pHLMs results in the formation of hydroxylated metabolites (analyzed as methylated derivatives), with para-hydroxylated metabolites being the major metabolites. (A) Metabolism scheme showing the chemical structure and abbreviations of PCB 95 metabolites (only one atropisomer is shown for clarity reasons). Gas chromatograms of (B) PCB 95 metabolite standards and (C) metabolites formed in an incubation with pHLMs (50 μM PCB 95, 90 min, 37 °C, 0.3 mg/mL protein, and 1 mM NADPH). (D) Relative formation rates of OH-PCB metabolites (relative peak area/nmol P450/min ×10²) in incubations with pHLMs and single donor HLMs (H1 to H5). Data are the mean ± standard deviation, n = 3.