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. 2004 Oct;72(10):5983–5992. doi: 10.1128/IAI.72.10.5983-5992.2004

FIG. 6.

FIG. 6.

Conjugation and salt resistance phenotypes of dotU and icmF mutants. (A) The following L. pneumophila strains were assayed for the ability to transfer an RSF1010 plasmid to E. coli recipient cells: wild-type strain Lp02 (column 1), dotA mutant strain Lp03 (column 2), ΔdotU strain JV1116 (column 3), ΔicmF strain JV1179 (column 4), and ΔdotU ΔicmF strain JV1181 (column 5). The conjugation frequency was calculated as the number of E. coli recipients per L. pneumophila donor cell. Assays were done in triplicate. Error bars indicate the standard deviations of the means. (B) The plating efficiency on 0.65% NaCl was determined for strains used in panel A, and is shown here as the percentage of NaCl-resistant CFU in a cell population. The strains were wild-type Lp02 (column 1), dotA mutant Lp03 (column 2), the ΔdotU strain JV1116 (column 3), the ΔicmF strain JV1179 (column 4), and the ΔdotU ΔicmF strain JV1181 (column 5). Assays were done in triplicate. Error bars indicate standard deviations of the means.