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. 2004 Oct;72(10):5983–5992. doi: 10.1128/IAI.72.10.5983-5992.2004

FIG. 8.

FIG. 8.

Suppression of ΔdotU ΔicmF intracellular growth defect by overexpression of DotH. (A) The wild-type L. pneumophila strain Lp02 (squares), the dotA mutant Lp03 (diamonds), and the ΔdotU ΔicmF strain JV1181 containing either the empty vector pJB908 (circles), the dotU icmF complementing clone pJB1191 (triangles), or the dotHGF complementing clone pJB2132 (hatched squares) were assayed for growth in U937 cells. Mean numbers of CFU are plotted as a function of time. (B) The wild-type L. pneumophila strain Lp02 (squares), the dotA mutant Lp03 (diamonds), and the ΔdotU ΔicmF strain JV1181 containing either the empty vector pJB908 (circles), the dotU icmF complementing clone pJB1191 (triangles), or the dotH complementing clone pJB1555 (hatched squares) were assayed for growth in U937 cells. (C) The wild-type L. pneumophila strain Lp02 (squares), the dotA mutant Lp03 (diamonds), and the ΔdotU ΔicmF strain JV1181 containing either the empty vector pJB908 (circles), the dotU icmF complementing clone pJB1191 (triangles), or the dotG complementing clone pJB1554 (hatched squares) were assayed for growth in U937 cells. (D) The wild-type L. pneumophila strain Lp02 (squares), the dotA mutant Lp03 (diamonds), and the ΔdotU ΔicmF strain JV1181 containing either the empty vector pJB908 (circles), the dotU icmF complementing clone pJB1191 (triangles), or the dotF complementing clone pJB2121 (hatched squares) were assayed for growth in U937 cells. Growth curves were determined in the presence of 100 μM IPTG. Assays were done in triplicate. Error bars indicate standard deviations of the means. d, day.