TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source | Deoxy- ribosea |
|---|---|---|---|
| Nonpathogenic E. coli strains | |||
| K-12 MG1655 | Nonpathogenic reference strain (genome sequenced) | 5 | − |
| MG1655 Rif | Spontaneous rifampicin-resistant derivative of MG1655 | This study | − |
| MG1655 Nal | Spontaneous nalidixic acid-resistant derivative of MG1655 | This study | − |
| EC185 | Isolate from feces of a healthy volunteer | 15 | + |
| ExPEC strains | |||
| AL862 | Human blood isolate | 18 | + |
| CFT073 | Isolate from blood of a patient with symptomatic pyelonephritis (genome sequenced) | 31 | + |
| CFT073deoK | CFT073 isogenic mutant with deoK gene deleted | This study | − |
| CFT073 Nal | Spontaneous nalidixic acid-resistant derivative of CFT073 | This study | + |
| InPEC strains | |||
| 55989 | Enteroaggregative strain producing AAF-III fimbriae (tetracycline resistant) | 3 | + |
| 55989deoK | 55989 isogenic mutant with of deoK gene deleted | This study | − |
| AL866 | 55989deoK carrying pILL1314 | This study | + |
| AL868 | 55989deoK carrying pUC18 | This study | − |
| 55989 Nal | Spontaneous nalidixic acid-resistant derivative of 55989 | This study | + |
| AL867 | 55989 Nal carrying pUC18 | This study | + |
| 55989 Rif | Spontaneous rifampicin-resistant derivative of 55989 | This study | + |
| Plasmids | |||
| pILL1272 | 45-kb Sau3A fragment containing the deoK operon of AL862 cloned into pHC79 (carbenicillin resistant) | 18 | + |
| pILL1307 | pILL1272 containing a deoK gene mutated by partial deletion and insertion of the kanamycin resistance cassette (carbenicillin and kanamycin resistant) | This study | − |
| pILL1287 | 45-kb Sau3A fragment containing the deoK operon of 55989 cloned into pHC79 (carbenicillin resistant) | 3 | + |
| pUC18 | E. coli cloning vector (carbenicillin resistant) | 32 | − |
| pILL1314 | BamHI-KpnI PCR product corresponding to the deoK gene from 55989 inserted into pUC18 (carbenicillin resistant) | This study | − |
a
Growth on minimal medium supplemented with 0.1% deoxyribose and hybridized with probe A.