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. 2004 Oct;72(10):5814–5823. doi: 10.1128/IAI.72.10.5814-5823.2004

FIG. 1.

FIG. 1.

Plasmid pUTE29-plcR-papR contains the B. cereus plcR-papR region inserted into pUTE29. A derivative plasmid containing only papR was made by digesting with SmaI and SnaBI and ligating. A derivative plasmid containing only plcR was made by PCR amplification with primers Pf and Pr (locations indicated by arrows). The regions responsible for the replication of pUTE29-plcR-papR are shown by arrows and designated as rep pBC16 (for replication in B. anthracis) and rep pUC18 (for replication in E. coli).