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Stability of PlcR complexes. Whole-cell proteins from 12-h cultures of B. anthracis SdT2 and SdT12 were treated sequentially with DNase I and Triton X-100 or with DNase I and urea. Triton-containing samples were heated in SDS sample buffer, whereas urea-containing samples were loaded directly onto the gels. C-PlcR antiserum 1451 was used for the blotting analysis. M, molecular mass markers.
