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. 2004 Oct;72(10):5814–5823. doi: 10.1128/IAI.72.10.5814-5823.2004

FIG. 6.

FIG. 6.

Growth, PlcR-PapR production, and enzyme activities for B. anthracis strains. (A) Growth curves for strains incubated at 37°C in LB broth. (B) Western blot analysis of whole-cell proteins from the B. anthracis SdT12, using preadsorbed N-PlcR antiserum 1447. (C and D) PC-PLC (C) and SPH (D) activities of extracellular proteins. Equal amounts of extracellular protein (2 to 10 μg for PC-PLC and 0.1 to 0.5 μg for SPH) were assayed by the Red Amplex reagents. Maximum root-mean-square deviations did not exceed 10% for the PC-PLC (C) and SPH (D) determinations. Filled square, B. anthracis SdT1; filled diamond, B. anthracis SdT12.