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. 2004 Oct;72(10):5814–5823. doi: 10.1128/IAI.72.10.5814-5823.2004

FIG. 8.

FIG. 8.

Assay of cell lysates for PlcR proteins binding to DNA duplexes and cellular DNA. (Upper panel) The NoShift assay was performed as described above, using a double-stranded biotinylated T1 (final concentration, 0.5 pmol/μl) and increasing amounts of SdT, SdT1, SdT2, and SdT12 cell extracts. (Lower panel) PlcR proteins were analyzed by Western blot of whole-cell proteins from B. anthracis SdT, SdT1, SdT2, and SdT12 using C-PlcR antiserum 1451 as described above. The strains were grown for 10 h in LB broth at 37°C. M, molecular mass markers.