TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Relevant characteristic(s)a | Source or reference | ||
|---|---|---|---|---|
| Plasmids | ||||
| pUTE29-plcR-papR | B. cereus 569 plcR-papR DNA fragment inserted into pUTE29 | 16 | ||
| pUTE29-plcR | pUTE29-plcR-papR without papR gene | This work | ||
| pUTE29-papR | pUTE29-plcR-papR without plcR gene | This work | ||
| pFP12 | pUTE29-plcR fusion to papR | This work | ||
| pAE5 | Encodes B. cereus 569 plcR under control of B. anthracis protective antigen gene promoter | 16 | ||
| Strains | ||||
| B. anthracis | ||||
| Sterne 34F2 DeltaT (=SdT) | Sterne 34F2 cured of pXO1; therefore, pXO1−pXO2− | 6 | ||
| Ames 33 | pXO1− pXO2− Ames derivative strain | 16 | ||
| SdT1 | SdT electroporated with pUTE29-plcR-papR; Tcr; does not produce intracellular PlcR; nonhemolytic strain | 16 | ||
| SdT2 | SdT(pAE5); Kmr; produces intracellular B. cereus PlcR; weakly hemolytic | 16 | ||
| SdT5 | SdT electroporated with pUTE29-plcR; Tcr; does not produce intracellular PlcR; nonhemolytic | This work | ||
| SdT6 | SdT electroporated with pUTE29-papR; Tcr; does not produce intracellular PlcR; nonhemolytic | This work | ||
| SdT12 | SdT electroporated with pFP12; Tcr; produces intracellular PlcR-PapR fused protein; hemolytic | This work | ||
| A12 | Ames 33 electroporated with pFP12; Tcr; produces intracellular PlcR-PapR fused protein; hemolytic | This work | ||
| E. coli | ||||
| XL2-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lac1qZΔM15 Tn10 (Tcr) Amy Cmr] | Stratagene | ||
| SCS110 | rpsL (Smr) thr leu endA thi-1 lacY galK galT ara tonA tsx dam dcm supE44D (lac-proAB) [F′ traD36 proAB lac1qZΔMIS] | Stratagene |
a
Kmr, kanamycin resistant; Tcr, tetracycline resistant.