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Apoptotic gene array analysis with RNA from THP-M cells infected with E. coli. Gene array membranes containing 23 apoptotic genes including β-actin and GAPDH are screened by using the RNA isolated from THP-M cells infected with either OmpA+ (A) or OmpA− (B) E. coli for 1 h; a nonradioactive detection method was used, and the blots were exposed to X-ray film and scanned. The densities of the spots were calculated by using the NIH software Image J.
