FIG. 4.

Increased expression of Bcl_XL in OmpA⁺ E. coli-infected macrophage cell lines. Confluent monolayers of either THP-M (A) or RAW 264.7 (B) cells were treated with ST with or without prior infection with either OmpA⁺ or OmpA⁻ E. coli. In separate experiments the cells were infected with bacteria only. Total cell lysates were prepared, separated by SDS-10% polyacrylamide gel electrophoresis, and subjected to immunoblotting with anti-Bcl_XL, anti-Bax, or anti-β-actin antibodies. Total cell lysates from untreated cells were used as controls. (C and D) The blots were scanned and plotted by using arbitrary units of the densities that are normalized to the densities of the respective β-actin bands. The densities of bands in the control are taken as 100%.