FIG. 6.

Detection of mitochondrial transmembrane potential in E. coli-infected RAW 264.7 cells by using MitoCapture. RAW cells were seeded in 8-well chamber slides and cultured to confluence. The cells were left untreated (A to C), treated with ST (D to F), or infected with either OmpA⁺ E. coli (G to I), OmpA⁺ E. coli plus ST (J to L), or OmpA⁻ E. coli plus ST (M to O). Cells were washed and further incubated with diluted MitoCapture solution for 15 min at 37°C in a CO₂ incubator. After further washing, the cells were fixed in 2% paraformaldehyde solution for 10 min at room temperature, washed again, and mounted with a coverslip with antifade solution.