FIG. 9.

Complementation of OmpA⁻ E. coli with a plasmid containing the ompA gene suppresses the activation of caspases in THP-M cells. Confluent monolayers of THP-M cells were infected with OmpA⁻ E. coli transformed with either a plasmid containing the ompA gene (pOmpA⁺ E. coli) or plasmid alone (pOmpA⁻ E. coli) for 1 h. In some experiments the infected cells were treated with ST for an additional 3 h. Total cell lysates were prepared and subjected to Western blotting with anti-caspase 3, anti-caspase 9, or anti-PARP antibodies.