FIG. 3.

Flow cytometric analysis of Gr-1⁺ cells in the spleens of A/J and C57BL/6J mice during C. albicans infection. Spleens were removed from control and C. albicans-infected A/J and C57BL/6J (A/Jinf. And B6inf., respectively) mice 24 h postinfection. Spleen cell preparations were stained with R-phycoerythrin-conjugated rat anti-mouse Gr-1 antibody. The percentage of cells positive for R-phycoerythrin fluorescence was determined by flow cytometry. The standard error of the mean is indicated for each sample. Numbers in parentheses indicate the number of mice analyzed per experimental group. Asterisks indicate statistically significant differences (P = 0.005).