TABLE 1.
Classification of bacteria in wild-type and CF mouse small intestines by sequencing of cloned 16S PCR products
| Classificationa | No. of clones
|
|
|---|---|---|
| Wild-type mice | CF mice | |
| Uncultured bacteriab | 37 | 2 |
| Klebsiella oxytoca, Klebsiella pneumoniae, and Klebsiella granulomatis | 20 | 0 |
| Enterobacter aerogenes or Enterobacter asburiae | 13 | 0 |
| Other Enterobacteriacaec | 5 | 95 |
| Lactococcus lactis | 9 | 0 |
| Acinetobacter sp. | 5 | 0 |
| Pantoea sp. and Pantoea agglomerans | 4 | 0 |
| Unclassified β-Proteobacter | 2 | 0 |
| Helicobacter typhlonicus | 1 | 1 |
| Bacteroides acidofaciens | 1 | 0 |
| Streptococcus gordonii or Streptococcus parasanguinis | 1 | 0 |
| Clostridium perfringens | 0 | 6 |
a
When the 16S sequence was definitive, both genus and species are listed. When more than one species matched the sequence data or more than one species was unequivocally identified, all species are listed.
b
The wild-type group includes five clones that did not match anything in the GenBank database even though the cloned PCR product inserts were of the appropriate size for the 16S primers used (460 to 475 bp).
c
The sequences of the 16S PCR products in this group are identical between various family members (E. coli, Escherichia fergusonii, Escherichia vulneris, Photorhabdus liminescens, and Shigella flexneri).