TABLE 1.
Strains used for Western blot, immunoprecipitation, immunoelectron microscopy, and infant mouse protection assays
| Strain | Species | TCP |
|---|---|---|
| O395 | V. cholerae O1 classical | Wild-type TCP |
| RT4031 | V. cholerae O1 classical | TCP negative |
| RT4018 | V. cholerae O1 classical | P169→A |
| RT4056 | V. cholerae O1 classical | K172→A |
| RT4054 | V. cholerae O1 classical | D175→A |
| RT4506 | V. cholerae O1 classical | T180→A |
| RT4058 | V. cholerae O1 classical | H181→A |
| RT4060 | V. cholerae O1 classical | E183→A |
| RT4055 | V. cholerae O1 classical | K184→A |
| RT4062 | V. cholerae O1 classical | K187→A |
| RT4469 | E. coli Origami/pET15b- cl-TcpA clone | Wild-type TCP NH2 terminal His tag |