TABLE 2.

Differences in log CFU between the wild-type strain and B. melitensis 16M mutants in cellular infections

Conditions	Time postinfection (h)	Differences in bacterial countsa
		Bovine macrophages			HeLa cells
		exbB mutant	dstC mutant	pth/dugA mutant	exbB mutant	dstC mutant	pth/dugA mutant
Iron rich	48	0.18 ± 0.16	0.31 ± 0.34	−0.18 ± 0.50	−0.32 ± 0.10	0.01 ± 0.13	−0.15 ± 0.01
Iron restricted	48	0.17 ± 0.03	NDb	ND	0.33 ± 0.12	ND	ND

^aDifferences were calculated as follows: (total mean log CFU of wild type) − (total mean log CFU of mutant). The experiments were performed with bovine macrophages and HeLa cells at 48 h postinfection, and bacterial inocula were grown in iron-rich or iron-restricted conditions. The data are means ± standard deviations; the standard deviations for the wild-type strain were ±0.18 and ±0.20 for the two cellular models tested. Cellular infection experiments were performed twice in triplicate.

^bND, not determined.