TABLE 4.
Liquid growth assay in iron-poor medium (LB medium with 500 μM DFA) for utilization of the DHBA siderophore (500 μM) by B. melitensis wild-type strain 16M and the exbB mutant as controls and the pth/dugA mutant transformed with pBBRdugA1 and pBBRdugA2
| Medium | Growth of strainsa
|
|||
|---|---|---|---|---|
| exbB | Wild type | pth/dugA with pBBRdugA1 | pth/dugA with pBBRdugA2 | |
| Iron-rich medium (LB medium) | + | + | + | + |
| Iron-poor (LB medium with DFA) | − | + | + | − |
| Iron-poor medium with DHBA | − | + | + | − |
a
+, growth equivalent to wild-type growth; −, growth much less than wild-type growth. Growth was examined 48 h after inoculation.