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. 2017 Jan;1861(1Part A):3154–3163. doi: 10.1016/j.bbagen.2016.07.020

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 8 — NMR TROSY spectra and Ile side-chain conformational distributions. (A) Methyl TROSY spectra of 100 μM U-[15N, 2H], ILV^CH3 IscU-Zn (black spectrum) was titrated with 2 molar equivalences of U-[2H] IscS (red spectrum) and then with 2 molar equivalences of U-[2H] CyaY (green spectrum). The assignments of the Ileδ1, Leuδ1/2 and Valγ1/2 for free IscU-Zn are shown. (B) Distributions of the Cα-Cβ dihedral angle of selected IscU isoleucines from the binary system simulation. Each plot reflects the conformation of both isoleucines of the two protomers.