Fig. 4.

Impact of systemic TNF-α on sickness behavior in NBH and ME7 animals. Sickness behavior was examined following TNF-α (250 μg/kg) i.p. administration in ME7 prion diseased mice 18 weeks post-inoculation. Temperature change and body weight are presented as change from baseline. Rears and open field are graphed as percentage of baseline. Data were analysed using a three-way ANOVA followed by Bonferroni multiple comparison test comparing each treatment group to its relevant control group. Statistical significance is denoted by ^∗(P < 0.05), ^∗∗(P < 0.01), ^∗∗∗(P < 0.001). All data are represented as the mean ± SEM, n = 6–15 for all groups.