TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) | Novagen |
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) | Gibco BRL |
| XL1-Blue | relA1 lac [F′ proAB laclqZΔM15 Tn10 (Tetr)] | Stratagene |
| S. pneumoniae | ||
| CP1200 | Nonencapsulated derivative of Rx1; malM511 str-1 | Choi et al. (6) |
| HYK2 | CP1200 ΔclpP::ermB | Kwon et al. (15) |
| D39 | Encapsulated; type 2 | Avery et al. (1) |
| HYK302 | D39 ΔclpP::ermB | Kwon et al. (15) |
| Plasmids | ||
| pET30(a) | 5.4 kb; Apr | Novagen |
| pKHY002 | 6.0 kb; histidine-tagged clpP in pET30(a) | This study |