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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Cell Mol Bioeng. 2010 Dec 14;4(1):81–90. doi: 10.1007/s12195-010-0153-8

Figure 7.

Figure 7

Alizarin red de-staining results using 10% cetylpyridinium in sodium phosphate relative to pulsed ultrasound treated cultures. Alizarin red MC3T3-E1 cultures were destained and quantified at OD562nm. Significant increase (33.74 ± 8.37%) in matrix calcification was observed at day 18 in 100 kHz pAMUS stimulated samples relative to LIPUS treated samples. 45 kHz samples didn't show significant increase (*p < 0.005, n = 3). Statistical significance was calculated using student t-test and one-way ANOVA; error bars represent ± standard deviation.