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. 2004 Oct;72(10):5832–5839. doi: 10.1128/IAI.72.10.5832-5839.2004

FIG. 1.

FIG. 1.

BFT specifically stimulates IL-8 secretion by HT29/C1 cells that is not serum dependent. (A) Subconfluent HT29/C1 cells were treated with BFT (100 ng/ml, for 18 h), boiled BFT, B. fragilis LPS (5 μg/ml), E. coli LPS (5 μg/ml), or PMA (1 μg/ml) in DMEM with 2% FBS. Secreted IL-8 was measured in the culture medium as described in Materials and Methods. P < 0.01, BFT versus untreated control HT29/C1 cells (n = 8 experiments); P < 0.01, PMA versus untreated control HT29/C1 cells (n = 3 experiments). (B) HT29/C1 cells were treated with BFT (100 ng/ml, for 4 h) or BFT buffer alone, and the level of IL-8 was measured in the culture medium as described in Materials and Methods. P ≤ 0.05 or 0.01, BFT versus control in the presence or absence of 2% FBS, respectively (N = 3 to 7 experiments per condition).