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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Immunol. 2016 Nov 30;198(1):443–451. doi: 10.4049/jimmunol.1601179

Figure 2. IL-1 dependent effects of MSU crystals on the inflammatory profile of term placental explants.

Figure 2

In human term placental explants, IL-1β (A), MCP-1 (B) and IL-6 (C) secretion were all induced by MSU crystals and abrogated with the use of the caspase-1 inhibitor. The specificity to the IL-1 pathway was confirmed using the IL-1 receptor antagonist (IL-1Ra) which also decreased the secretion of IL-1β (D), MCP-1 (E) and IL-6 (F). Increased caspase-1 activity was detected in the supernatant (G) alongside increased cleaved caspase-1 in the supernatant and decreased in the lysate detected by western blot (representative example shown in H). (n=4-6) Results presented as mean ± SEM. Statistical analysis by one-way ANOVA with Dunnett’s multiple comparison post-test (A-F) or t-test with welch correction (G). *p<0.05; **p<0.01.