Skip to main content
. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Immunol. 2016 Nov 30;198(1):443–451. doi: 10.4049/jimmunol.1601179

Figure 3. IL-1-dependent effects of MSU crystals of cytotrophoblast viability and syncytialization.

Figure 3

Term human cytotrophoblast exposed to MSU crystals (100μg/ml) showed increased number of M30+ apoptotic cells (brown staining in A) to a similar extent has exposure with IL-1β and this effect was dependent on IL-1 since blocking the IL-1 pathway using IL-1Ra protected cytotrophoblast against MSU crystals-induced cell death (A). Syncytialisation (addressed by staining for desmoplakin and quantifying the percentage of multinucleated cells counted (white arrows)) was decreased by MSU crystals or IL-1β treatment (B). (n=4-6). Results presented as mean ± SEM. Statistical analysis by one-way ANOVA with Dunnett’s multiple comparison post-test. Scale bar: 50μm. *p<0.05, **p<0.01, ***p<0.001.