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. 2015 Sep 2;4(9):e54. doi: 10.1038/emi.2015.54

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Construction of the B. burgdorferi strain overexpressing EF-Tu. (A) Diagram of the shuttle vector pYY003 carrying a tuf gene under the control of a strong and constitutive flaB promoter. (B) Coomassie-stained gel showing overexpression of EF-Tu. Lane 1, wild-type strain B31-A3; lane 2, B31-A3/PflaB-tuf-HA. The band corresponding to EF-Tu was indicated by arrow. (C) Confirmation of over-production of HA-tagged EF-Tu by immunoblotting using either HA monoclonal antibody or antibody against FlaB (loading control). Lane 1, wild-type strain B31-A3; lane 2, B31-A3/PflaB-tuf-HA.