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. 2015 Sep 2;4(9):e54. doi: 10.1038/emi.2015.54

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B. burgdorferi EF-Tu is a soluble protein associated with the PC fractions. (A) Immunoblot analyses of detergent-enriched (Det) and aqueous-enriched (Aq) phases of B. burgdorferi with antibodies against EF-Tu or OspA. (B) Immunoblot analyses of PC fraction and outer membrane vesicles (OMVs) of B. burgdorferi separated by sucrose density gradient centrifugation. Whole cell lysates of wild-type B. burgdorferi (WT) were used for comparison. Equal amounts of proteins were loaded and immunoblotted with antibodies directed against EF-Tu or OspA.