Fig. 1.

Biodistribution studies of m-NC-111In in CT26 tumor-bearing BALB/c mice under the influence of a magnetic field. Mice were intravenously injected with NC-¹¹¹In or m-NC-¹¹¹In at SPION content of 0-500 mg/kg. Blood samples were collected at specified time points and organs and tumors were excised at 24 h post-injection. (a) Scatter plots of % ID of m-NCs in tumor tissue (24 h) without or with magnetic targeting as a function of natural log of SPION injection dose. The best-fit linear regression line is shown with a correlation coefficient (r2) of 0.9338 in tumor tissue upon magnetic targeting (p = 0.0337). (b) Blood circulation of NC 1-5 with an increasing amount of SPION. (c) Liver and spleen accumulation profiles of NC 1-5 at 24 h post-injection with an increasing amount of SPION. Results are expressed as % ID/g of organ as mean ± SEM (n=3). The linear regression in (a) was performed using Graphpad Prism 5.0. One-way ANOVA was performed using IBM SPSS version 20 followed by Tukey’s multiple comparison test (**p < 0.01).