Fig. 3.

Experimental timeline of fibered confocal fluorescence microscopic (FCFM) imaging of m-NCs in tumor vasculature under the influence of a magnetic field (MF). CT26 tumor-bearing BALB/c mice were injected intravenously with m-NC-DiR and a magnet was applied only at one of the bifocal tumors (TU+) for 1 h. FCFM imaging was performed at 1, 4 and 24 h post-injection in both TU+ and TU-. Non-previously injected mouse was used for each time point. FITC-Dextran was injected intravenously at a dose of 500 mg/kg prior to each imaging time point as a macromolecular contrast agent. Inset (top) shows the dynamic FCFM imaging of m-NCs in tumor blood vessels during MF application to demonstrate the interaction of m-NCs and MF on a microscopic scale.