Figure 5.

Growth kinetics in Lats2^−/− MEFs. (A) Growth curve representative of passage 2 MEFs derived from wild-type (▪) and two independently derived Lats2^−/− MEF lines (▴ and •). (B) Representative saturation density of growth for passage 2 MEFs derived from wild-type (•) and two independently derived Lats2^−/− MEF lines (▴ and ▪). (C) Plating efficiency at low seeding density (colony formation/3000 cells plated) for two wild-type MEF lines (grey bar, n=3) and two Lats2^−/− MEF lines (black bar, n=3). (D) Representative determination of contact inhibition of growth. A total of 5 × 10⁴ cells of either wild-type or Lats2^−/− MEFs were plated in 6 cm dishes. Cells were maintained in culture without passage for 2 weeks and stained with crystal violet. (E, F) Morphology of wild-type MEFs (E) and foci observed in Lats2^−/− MEFs (F).