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. 2016 Oct 20;15(23):3268–3277. doi: 10.1080/15384101.2016.1242537

Figure 1.

Figure 1.

Typical responses of the prepared HeLa cell lines to paclitaxel. (A) Time-lapse observation of MOMP-prone (GI23) and MOMP-reluctant (#44) HeLa cells treated with 10 nM paclitaxel. Contrast of bright field images (BF) is computer-enhanced. The time after drug addition is indicated below the panels. Exit from mitosis was judged by the loss of GMMN-Venus fluorescence. Cells a and b (encircled with dotted lines) of GI23 terminated metaphase arrest with MOMP, followed by loss of mCherry fluorescence (CF) and blebbing (white arrows). Cells e and f of line #44 slipped out of metaphase arrest and underwent blebbing (white arrows) while retaining mCherry fluorescence until the end of observation. (B) Switch-like loss of mCherry fluorescence in each of 4 GI23 cells treated with 10 nM paclitaxel. Cells a and b are identical to those shown in (A). (C). The number of cells retaining mCherry fluorescence is plotted against time after addition of 10 nM paclitaxel. All cells in a single microscopic field were counted for each respective cell line. The slight increase in the number of #44 cells is due to mitosis of the minor fraction.