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. 2016 Oct 18;15(23):3296–3305. doi: 10.1080/15384101.2016.1243630

Figure 1.

Figure 1.

Expression of Bcl2l10 protein during mouse oocyte maturation and localization of Bcl2l10, Tpx2, and Aurka in MI oocytes. (A, B) Western blot analysis of Bcl2l10 protein expression during normal in vitro oocyte maturation. GV, GVBD, MI, and MII oocytes were collected after 0, 2, 8, and 16 h of in vitro culture, respectively. Protein lysates of 200 oocytes were loaded per lane. α-Tubulin was used as a loading control. The experiment was performed 3 times, and the data are presented as the mean ± SEM. (C) Bcl2l10 and Tpx2 co-localized on microtubules, but Aurka localized in the MTOC region in MI oocytes (upper lane). Oocytes were stained with Bcl2l10-, Tpx2- and Aurka-specific primary antibodies simultaneously (from left to right). DNA was counterstained with DAPI (blue channel). Bcl2l10 (FITC, green channel), Tpx2 (Rhodamine, red channel), Aurka (Alexa 647, pink channel), and merged images. Bar=10 µm. (Lower lane) Higher-magnification photographs of the corresponding image are shown below. Bar=5 µm.