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. 2016 Oct 18;15(23):3296–3305. doi: 10.1080/15384101.2016.1243630

Figure 2.

Figure 2.

Bcl2l10 is a binding partner of Tpx2 but not Aurka. (A, B) Co-immunoprecipitation assays were performed using lysates from HEK293T cells that were co-transfected with the indicated plasmid constructs. Cell lysates were precipitated with an anti-Flag antibody and blotted with either an anti-Myc or anti-Flag antibody, as indicated. (A) Flag-Bcl2l10 and Myc-Tpx2 plasmids were co-transfected into HEK293T cells. (B) Flag-Bcl2l10 and Myc-Aurka plasmids were co-transfected into HEK293T cells. (C) Bcl2l10 forms a complex with Tpx2 in mouse oocytes. Lysates from 150 MII oocytes were used as the input (lane 1). Protein extracts from 1,000 MII oocytes were immunoprecipitated with goat IgG (lane 2) or a Bcl2l10 antibody (lane 3) and reacted with Tpx2 and Aurka antibodies.