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. 2016 Oct 20;15(23):3251–3267. doi: 10.1080/15384101.2016.1242534

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© 2016 Taylor & Francis

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Figure 4. — LDOC1 inhibits GNL3L function. (A) MTT and (B) Annexin-V binding assays were carried out after 48 hrs of transfection of HEK293T cells with Flag-GNL3L, LDOC1-HA or corresponding control vectors. HEK293T cells were transfected with Flag-GNL3L or control vector along with LDOC1 esiRNA or control esiRNA. After 48 hrs of transfection, (C) Annexin-V binding as well as (D) MTT assays were performed. The knockdown of LDOC1 was confirmed by RT-qPCR analysis using specific primers. (E) The expression of Flag-GNL3L was analyzed by western blot analysis using anti-Flag antibody.